The Osceola & St Croix Valley Railway is a heritage railroad in Osceola, Wisconsin. It is operated by the Minnesota Transportation Museum on former Soo Line trackage.
The railway uses three diesel locomotive, 10 coaches and two cabooses to provide train excursions for 20,000 passengers per year. The railway has operated from Osceola since 1992.
The railway operates excursions on weekends and holidays, April through October, plus special events. This route follows the St. Croix valley for 10 miles (16 km), a round-trip taking 90 minutes. Then, after a 30 minute break, the train goes 5 miles (8.0 km) to Dresser, Wisconsin.